Molecular Characterization and Haplotype Analysis of Low Phytic Acid-1 (lpa1) Gene Governing Accumulation of Kernel Phytic Acid in Subtropically-Adapted Maize

Maize is an important food, feed, fodder and industrial crop in addition to being a valuable source of micronutrients. Phytic acid (PA), anti-nutritional factor maize, makes crucial minerals inaccessible monogastric animals. The low phytic acid-1 (lpa1) gene located on chromosome-1S 7292 bp long with 11 exons, the recessive lpa1-1 allele reduces accumulation PA thereby enhances bioavailability essential maize kernels. Here, we characterized full-length Lpa1 sequence three mutants (lpa1-1) seven wild-type (Lpa1) inbreds. Sequence analysis revealed 607 polymorphic sites across sequences, indicating wide variability for among Further, SNP from “C” “T” differentiated mutant-type alleles at 1432 amino position. Gene-based diversity 48 diverse inbreds using 15 InDel markers formation 42 distinct haplotypes; six which (Hap6, Hap16, Hap17, Hap19 Hap27 Hap31) were shared by more than one genotype. number exons ranged 11–19 genotypes 6–14 26 orthologues. Major functional motifs detected ATP-binding Cassette (ABC) transporter trans-membrane region ABC transporter. Phylogenetic tree nucleotide protein sequences closer relationship Sorghum bicolor, Panicum hallii, Setaria italica S. viridis. This study offered newer insights into understanding genetic related crop-species, information generated here would further help exploiting lpa1 mutant enhancement nutritional value